Development of a real-time NASBA for respiratory syncytial virus
نویسندگان
چکیده
منابع مشابه
Advancing Drug Development for Respiratory Syncytial Virus
Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections (LRTIs) in infants and children around the world, resulting in three million hospitalizations and several hundred thousand deaths annually, the vast majority of which occur in developing nations. Treatment options for RSV are limited, and efforts to produce a vaccine have been unsuccessful thus far. Giv...
متن کاملSensitive Commercial NASBA Assay for the Detection of Respiratory Syncytial Virus in Clinical Specimen
The aim of the study was to evaluate the usability of three diagnostic procedures for the detection of respiratory syncytial virus in clinical samples. Therefore, the FDA cleared CE marked NOW(R) RSV ELISA, the NucliSENS EasyQ RSV A+B NASBA, and a literature based inhouse RT-PCR protocol were compared for their relative sensitivities. Thereby, NASBA turned out to be the most sensitive method wi...
متن کاملReal-Time NASBA
NASBA is an isothermal nucleic acid amplifi cation method that is particularly suited to detection and quantifi cation of genomic, ribosomal or messenger RNA. The product of NASBA is single-stranded RNA of opposite sense to the original target. The fi rst developed NASBA methods relied on liquid or gel-based probe-hybridisation for post-amplifi cation detection of products. More recently, real-...
متن کاملIdentification of Respiratory Syncytial Virus (RSV) Genome in the Stool of a Child with Acute Gastroenteritis
Case Report: Some viruses have been reported to cause respiratory and gastroenteric infections simultaneously. In this case we present isolation of human respiratory virus (RSV) type B from diarrheal sample of a 12 months' child with acute gastroenteritis. Results: The results indicated the presence of RSV subtype B genome in all three stool samples. Moreover, no sign of co-infections with oth...
متن کاملRapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology.
The performance of a sensitive and specific qualitative respiratory syncytial virus (RSV) assay based on NASBA technology and real-time molecular beacon detection is presented. Very low detection limits for both RSV A and RSV B were determined: 95% detection hit-rate of 95 and 47 copies/input in isolation for RSV A and RSV B, respectively. RSV was detected in a wide variety of clinical samples ...
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ژورنال
عنوان ژورنال: Journal of Clinical Virology
سال: 2006
ISSN: 1386-6532
DOI: 10.1016/s1386-6532(06)80767-5